1):-GLUCOSE AGAR DEEPS
The organism is inoculated into the molten medium at 45°C. The culture is then allowed to solidify, and incubated at 37 °C. Growth of the anaerobic organisms takes place below the surface of the medium.
Long thin tubes are half-filled with this medium, boiled for 5 min to remove dissolved oxygen, and the surface of the medium is covered with sterile molten Vaseline. This seals the medium from the air, and inoculation is carried out through the layer of Vaseline by means of a pasteur pipette.
Heated to redness, cooled, and added to liquid media, iron strips will oxidize in the medium, and produce anaerobic conditions.
Rabbit kidneys are rich in catalase and can thus remove the peroxide formed by growing anaerobic organisms under aerobic conditions. The medium consists of fresh sterile rabbit kidney covered with a long column of ascitic fluid on which a layer of Vaseline is poured. The tubes are inoculated through the Vaseline surface by means of a pasteur pipette. The last method is rarely used but is included here to illustrate the principles involved.