This Gram-positive motile bacillus is very widely distributed in nature for example in soil and in the intestines of animals, and is generally regarded as a harmless saprophyte. It is about 6-8 μm by 0-8 μm and Gram-negative forms are frequent in older cultures. Its oval, central or subterminal spores may be highly resistant and the organism is frequently encountered in mixed cultures in the laboratory, even after preliminary heating of these cultures to select heat-resistant pathogens. Its spores may survive boiling for periods of from 15 minutes up to 6 hours. Clostridium sporogenes is frequently isolated from wound exudates in association with accepted pathogens. Whilst its presence may accelerate an established anaerobic infection by enhancing local conditions, it does not by itself cause gas gangrene and cannot be regarded as a pathogen in its own right.