Diagnosis Of Opportunistic Infections.
The diagnosis is based on :-
Collect fluid using capillary tubes and block both ends. Take the specimen to virology lab. Inoculate the material into suitable host for example, chorio allantoic membrane of 12 day old.
b). Electron microscopy
put some of the fluid and examine them under electron microscopy.
stain the biopsyand look for cytopathic changes such as intranuclear inclusion bodies.
Take acute and convalescence sera for a period of 2-4 hours. If there is rise in titer 4x its diagnostic.
Hepatitis B Virus
Can be diagnosed using :-
a). Electron microscopy
b). Serological test. Most commonly used at present is a passive haemagglutination test. In these test, red blood cells are coated with HBS- antibody (from serum of convalescent patient) are exposed to the serum under test and are agglutinated if it contains HBsAg (Hepatitis B surface antigen).